Ronaldo, Kulusevski lead Juventus at Porto
Ronaldo, the competition's record goalscorer, will have his work cut out against a side who have not conceded a home goal so far in this season's tournament.
Coach Andrea Pirlo juggled Juve's defence with Alex Sandro returning, playing alongside veteran captain Giorgio Chiellini, Matthijs de Ligt and Danilo.
Juventus have never lost against Porto in the Champions League with three wins and a draw.
Both teams are two-time European Cup winners.
Juventus's last title was in 1996 and Porto's in 2004.
Starting line-ups for the Champions League last-16, first leg between Porto and Juventus at Estadio do Dragao on Wednesday (2000 GMT kick-off):
Agustin Marchesin; Wilson Manafa, Pepe, Chancel Mbemba, Sanusi Zaidu; Jesus Corona, Otavio, Sergio Oliveira, Andrea Mateus Uribe; Moussa Marega, Medhi Taremi
Coach: Sergio Conceicao (POR)
Coach: Andrea Pirlo (ITA)
Referee: Carlos del Cerro Grande (ESP)