First woman referee at Copa Libertadores
The group match between Argentine side Defensa y Justicia and Independiente del Valle of Ecuador on Thursday had an all-woman refereeing team.
Alves was supported by assistant referees Neuza Back, also from Brazil, and Cindy Nahuelcoy of Chile, with another Chilean, Maria Belen Carvajal, as the fourth official.
The Group A match in Florencio Varela near Buenos Aires ended in a 1-1 draw.
Alves had already achieved a landmark when she became the first woman to officiate at a major men's competition when she served as a fourth official at the World Club Cup in Qatar in February.